Assembly and sequencing of a cDNA covering the entire mouse α1(IX) collagen chain

Abstract

Type IX collagen, a member of the FACIT family of collagenous proteins, contains heterotrimeric molecules of distinct α1(IX), α2(IX) and α3(IX) chains. In this paper we describe the assembly and nucleotide sequence of a cDNA encoding the entire mouse α1(IX) collagen chain. The nucleotide sequence provides for the first time the complete primary structure of the mouse chain. Knowledge of the complete structure of mouse α1(IX) collagen will be useful for investigations of type IX collagen expression during normal mouse development and for the generation of transgenic mice with specific defects in this collagen. © 1994.