By in situ hybridization, 44-100% of the blood eosinophils from five patients with hypereosinophilia and four normal subjects exhibited intense hybridization signals for TNF-α mRNA. TNF-α protein was detectable by immunohistochemistry in blood eosinophils of hypereosinophilic subjects, and purified blood eosinophils from three atopic donors exhibited cycloheximide-inhibitable spontaneous release of TNF-α in vitro. Many blood eosinophils (39-91%) from hypereosinophilic donors exhibited intense labeling for macrophage inflammatory protein-1α (MIP-1α) mRNA, whereas eosinophils of normal donors demonstrated only weak or undetectable hybridization signals for MIP-1α mRNA. Most tissue eosinophils infiltrating nasal polyps were strongly positive for both TNF-α and MIP-1α mRNA. By Northern blot analysis, highly enriched blood eosinophils from a patient with the idiopathic hypereosinophilic syndrome exhibited differential expression of TNF-α and MIP-1α mRNA. These findings indicate that human eosinophils represent a potential source of TNF-α and MIP-1α, that levels of expression of mRNA for both cytokines are high in the blood eosinophils of hypereosinophilic donors and in eosinophils infiltrating nasal polyps, that the eosinophils of normal subjects express higher levels of TNF-α than MIP-1α mRNA, and that eosinophils purified from the blood of atopic donors can release TNF-α in vitro.
CITATION STYLE
Costa, J. J., Matossian, K., Resnick, M. B., Beil, W. J., Wong, D. T. W., Gordon, J. R., … Galli, S. J. (1993). Human eosinophils can express the cytokines tumor necrosis factor-α and macrophage inflammatory protein-1α. Journal of Clinical Investigation, 91(6), 2673–2684. https://doi.org/10.1172/jci116506
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