An adaptation of dapi-banding to fishes chromosomes

Abstract

This is the description of a method to obtain DAPI-banding in fishes' chromosomes by its denaturation using formamide. The slides are aged in an incubator and dehydrated in an ethanol series. The next step is the denaturation in formamide 70% and staining with DAPI. The pattern obtained is mostly C-band like, but matches only partially with the C-band pattern usually found by Barium Hydroxide technique. The bands obtained also do not match with the band pattern by CMA3. These data and a review of literature bring the conclusion that the banding is related to the DNA base composition instead of being related to the chromosome structural changes resulting from a long denaturation process. © 2006 Taylor and Francis Group, LLC.