Studies on L-Glutamic Acid Fermentation Part II. Fermentative Production of L-Glutamic Acid from Glucose by Brevibacterium divaricatum nov. sp.

Abstract

Basic researches performed with the purpose of finding a better industrial method to produce L-glutamic acid has resulted in the successful isolation of Brevibacterium divaricatum nov. sp. The conditions of fermentation production of L-glutamic acid employing Brevibacterium divaricatum have been examined. After a series of experiments with moderate culture constituents under proper conditions for fermentation, it became possible to obtain 45mg/ml broth of L-glutamic acid after 30 hours' incubation taking glucose as the substrate, feeding a proper amount of urea aqueous solution during the process in order to keep the culture medium weak alkaline, offering nitrogen at the same time. The yield of L-glutamic acid was exceeded 45 % on the base of initial glucose supplied. All experimental details are discussed in this paper. © 1960, Japan Society for Bioscience, Biotechnology, and Agrochemistry. All rights reserved.