Gene conversion maintains nonfunctional transposable elements in an obligate mutualistic endosymbiont

Abstract

Long-term bacterial endosymbionts typically exhibit reduced genomes, lack genes encoding recombination functions and transposable elements, such as insertion sequences (ISs). In sharp contrast, I found that ISs constitute 2.4% of the genome of the obligate mutualistic endosymbiont Wolbachia wBm. Although no IS copy is transpositionally functional, I show that ISs persist in wBm because of frequent recombinational gene conversion (GC) homogenizing homologous IS sequences. These results not only indicate that there exists a functional recombination molecular machinery in wBm, but they also suggest that, by slowing down the rate of IS degradation and loss, GC may represent a major force influencing reductive evolution in wBm. The Author 2009.