DNA base composition and genome size of the prokaryotic symbiont in Riftia pachyptila (Pogonophora)

Abstract

It has been proposed that Riftia pachyptila, a pogonophoran tube worm abundant at hydrothermal deep sea vents, metabolizes solely via a chemoautotrophic symbiosiols. The symbionts resemble sulfur oxidizing bacteria and form the specific 'trophosome' tissue. Samples of DNA purified from trophosome and vestimentum (muscle) tissues of R. pachyptila were comparatively characterized by thermal denaturation studies, and by analysis of renaturation kinetics. The results show that the great majority of trophosome DNA is homogeneous and prokaryotic with a base ratio of approx. 58 mol% G + C. Its genome size (genetic complexity) is typical of free-living bacteria. Approx. 5% of trophosome DNA appears to be invertebrate DNA equivalent to that found in the vestimentum tissue which lacks symbionts. © 1984.