Cytotoxicity activity of Liverwort (Marchantia Polymorpha L.)Methanolic Extract to HeLa cell Line

Abstract

Background: Liverwort (Marchantia polymorpha L) is one of plant that has high active compound such as flavonoid, terpenoid, phenolic that has potential as anticancer. This research aimed to determine the effect of liverwort extract to viability and apoptosis induction. Method: Liverwort’s leave were extracted using methanol solvent. HeLa cell line is used as model of treatment. Cell were incubated 72 hours in RPMI-1640 and then treated with various concentration of liverwort’s extract. Active compound of liverwort assay using Thin layer Chromatography (TLC) method, viability/citotoxicity Hela cell using WST-1 method, apoptosis cell using fluoresen microscop. Analyz data used kruskawallis. Results:Liverwort active compound concist of flavonoid, terpenoid and phenolic. Concentration 150, 250, 500, 1000 μg / mL extract methanolic marchantia polymorpha inhibit 40 %, 67 %, 67 % and 79 % growth of Hela cell line. Cell apoptosis induction begins at a concentration of 50 μg / mL, whereas at a concentration of 250 μg / mL cell apoptotic phase of almost 100%. Conclusion: Methanolic extract of liverworts (M. polymorpha L.) inhibits growth of Hela cell and induced cell apoptotic.