Clindamycin-based 3D-printed and electrospun coatings for treatment of implant-related infections

Abstract

This study presents the development and characterisation of two novel bioactive coatings deposited on TiAlV and AISI 316LVM substrates. The coatings were prepared using 3D printing and electrospinning. The 3D-printed coating consisted of the cellulose nanofibril suspension, algi-nate, and carboxymethylcellulose (CMC), while CMC and polyethylene oxide were used to prepare the electrospun coating. Both coatings were loaded with the antibiotic clindamycin (CLIN), which is a bacteriostatic lincosamide known for its activity against streptococci, staphylococci, pneumo-cocci, Bacteroides species, and other anaerobes. Initial characterisation of the coatings was per-formed by attenuated total reflectance Fourier transform infrared spectroscopy, field emission scanning electron microscopy, and atomic force microscopy. Furthermore, the contact angle measure-ments, swelling rate, and biodegradability of the coatings were investigated. The released concentration of CLIN in PBS (pH = 7.4 at 25 °C) was determined by UV-VIS spectrophotometry. The coat-ings’ biocompatibility was determined using an MTT (3(4,5 dimethylthiazolyl-2)-2,5-diphenylte-trazolium bromide) assay using an osteoblast cell culture (hFOB 1.19, ATCC CRL 11372).