Analysis of Nitric Oxide from Chemical Donors Using CMOS Platinum Microelectrodes

Abstract

Electrochemical detection of NO generated from chemical donors is reported. Because NO is an important biological messenger, many donor sources and detection methods have been developed. Few reports have characterized NO donors using electrochemistry despite electrochemical techniques being sensitive and selective. Here, a CMOS platinum microelectrode array is interfaced with a microfluidic device for the electrochemical analysis of NO from (Z)-1-[N-(2-aminoethyl)-N-(2-ammonioethyl)amino]diazen-1-ium-1,2-diolate (DETA/NO). The donor parent amine fouls the electrode, resulting in substantial signal loss, but an electrochemical cleaning method was developed that substantially reduces fouling and allows detection of NO between 90nM and 1μM.