Expression pattern of Nogo-A, MAG, and NgR in regenerating urodele spinal cord

Abstract

Background: The mammalian central nervous system is incapable of substantial axon regeneration after injury partially due to the presence of myelin-associated inhibitory molecules including Nogo-A and myelin associated glycoprotein (MAG). In contrast, axolotl salamanders are capable of considerable axon regrowth during spinal cord regeneration. Results: Here, we show that Nogo-A and MAG, and their receptor, Nogo receptor (NgR), are present in the axolotl genome and are broadly expressed in the central nervous system (CNS) during development, adulthood, and importantly, during regeneration. Furthermore, we show that Nogo-A and NgR are co-expressed in Sox2 positive neural progenitor cells. Conclusions: These expression patterns suggest myelin-associated proteins are permissive for neural development and regeneration in axolotls. Developmental Dynamics 242:847-860, 2013. © 2013 Wiley Periodicals, Inc. Key Findings: Myelin associated factors (MAFs) are expressed in axolotl spinal cord during development and adulthood. Both Nogo-A and NgR are expressed in neural progenitor cells during spinal cord regeneration. In contrast to mammals these MAFs are permissive for CNS development and regeneration in axolotl. © 2013 Wiley Periodicals, Inc.