Effects of Elevated Tetracycline Concentrations on Aerobic Composting of Human Feces: Composting Behavior and Microbial Community Succession

Abstract

The effects of antibiotics on aerobic composting are investigated by dosing of tetracycline (TC) in fresh human feces with sawdust as biomass carrier. Variability in process parameters such as temperature, pH, water-soluble carbon, germination index (GI) and dehydrogenase activity (DHA) are evaluated at TC dosages of 0, 100, 250 and 500 mg/kg in a 21-day composting. Moreover, microbial community succession is examined by high-throughput 16S rRNA gene sequencing. Findings indicate significant impacts to the process parameters with the increase of TC concentration such as inhibition of temperature increases during aerobic composting, lowering of pH, increasing of water-soluble carbon residue, a decrease of GI, and hindering of DHA. Furthermore, elevated TC concentrations significantly alter the microbial community succession and reduce the community diversity and abundance. Therefore, interference in microbial community structures and a hindrance to biological activity are believed to be the main adverse effects of TC on the composting process and maturity of the composting products.