HLA-A gene variation modulates residual function of the pancreatic b-cells in children with type 1 diabetes

Abstract

Aim of the study: The study aimed to analyze an association between the HLA-A gene variation and a risk of type 1 diabetes development and to evaluate the association of HLA class I and class II alleles with b-cell destruction. Material and methods: A group of 108 children with type 1 diabetes were genotyped in HLA-A, -DRB1, and -DQB1 genes using hybridization with oligonucleotides probes. Plasma C-peptide concentration was assessed by radioimmunoassay method. Results: No differences in allele HLA-A distribution between type 1 diabetes patients and healthy individuals were found. Among “low C-peptide”(< 0.28 pmol/ml) individuals, the frequency of HLA-A∗02 allele was 41.3%, whereas only one HLA-A∗26 allele was detected in this group (0.7%). Conversely, among “high C-peptide”(≥ 0.28 pmol/ml) probands the prevalence of A∗02 allele was 19.7% (Pc = 0.008, OR = 1.4, 95% CI: 1.2–1.7) and A∗26 10.5 % (Pc < 0.007, OR = 0.15, 95% CI: 0.02–0.9). Genotype analysis showed that A∗02/∗02 and A∗02/X children were more likely to have “low” C-peptide at the onset compared to those with non-A∗02/non-A∗02 genotype (p = 0.008, OR = 1.6, 95% CI: 1.3–2.0 and p = 0.015, OR = 1.4, 95% CI: 1.1–1.9, respectively). A02 phenotype individuals had lower median C-peptide (0.17 pmol/ml) than non-A02 patients (0.26 pmol/ml, p = 0.008). Median C-peptide was higher in the A26-positive group comparing to A26-negative (0.40 and 0.20, respectively, p = 0.04). No association between HLA class II and C-peptide levels was observed. Conclusions: HLA-A alleles are not associated with disease development nevertheless strongly influence a residual pancreatic b-cell function. The results suggest a different role of HLA class I and class II in type 1 diabetes pathogenesis.