Profil Fraksi Sitotoksik terhadap Sel Murine Leukemia P-388 dari Ekstrak Biji Honje (Etlingera elatior)

Abstract

The research characterization of cytotoxic fraction against P-388 leukemia murine cells from the extract honje (Etlingera elatior) seed have been reported. This research lead to isolation and characterization of cytotoxic compounds against P-388 leukemia murine cells from the extract of E. elantior seed. The extract of E. elantior seed was maserated by methanol, n-hexane, and ethyl acetate, respectively and estimated their cytotoxic activity against P-388 leukemia murine cell with 3- (4, 5-dimetiltiazol-2-yl) -2,5-difeniltetrazolium bromide (MTT) assay guided toxicity test against of shrimp Artemia salina Leach. Brine shirmp Lethality Test (BSLT) method. The active extracts will be separated by fractionation using column chromatography, , and for analyzing the purity of isolate will estimate by TLC. The chemical structure of pure isolate will be identified by spectroscopies data UV Vis, FTIR, and LCMS. The ethyl acetate extract from honje seed have cytotoxic activity by leukemia P- 388 cell with IC50 19.21 µg/mL. The fraction toxic as cytotoxic against P-388 leukemia murine cells with IC50 19.555 µg/mL is flavonoid compouds their is resveratrol, lapachol, apigenin, methylated chrysin, 6,2’- dihydroxyflavanone, 3-hydroxy-3,4’-dymethoxyflavone and 4’-hydroxy-5,7-dimethoxyflavanone