Antioxidant and Anti-Inflammatory Activity of Ethanol Extract Stem of Etlingera rubroloba A.D. Poulsen

Abstract

Background and Objective: Etlingera rubroloba A.D. Poulsen (E. rubroloba) is a plant endemic to South-East Sulawesi which is used empirically by local people as a pain reliever for joints and to increase endurance after typhoid fever. This study aimed to determine the antioxidant and anti-inflammatory activity of the ethanol extract stem of E. rubroloba. Materials and Methods: The stem of the ethanol extract of E. rubroloba with evaluated for antioxidants using the FRAP method and anti-inflammatory activity with the parameter level of Tumor Necrosis Factor-Alpha (TNF-α) in vivo by using 6 groups of rats, namely the normal, the negative (Na-CMC 0.5%), the positive control (Diclofenac sodium) and the ethanol extract group at doses of 200, 300 and 400 mg kg-1 b.wt. Results: The results of this study indicated that the antioxidant activity value of the IC50 ethanol extract was 12.720±0.12 µg mL-1 and ascorbic acid (vitamin C) as a standard control was 3.14±0.12 µg mL-1. The TNF-α normal group (7.83 pg mL-1), negative control (250.92 pg mL-1), positive control (123.66 pg mL-1), treatment group dose 200 (192.20 pg mL-1), 300 (97, 95 pg mL-1) and 400 mg kg-1 b.wt. (28.78 pg mL-1). Conclusion: This study concluded that the ethanol extract of the stem of E. rubroloba has a very strong antioxidant activity and is an anti-inflammatory which is the best in reducing levels of TNF-α at a dose of 400 mg kg-1 b.wt.