Synthesis and preclinical studies of [ 61Cu]-N-(2- hydroxyacetophenone)glycinate as a possible PET radiopharmaceutical

Abstract

[ 61Cu]-N-(2-hydroxyacetophenone)glycinate ([ 61Cu] NHAG) was prepared using in house-made NHAG ligand and [61Cu]CuCI 2 produced via the natZn(p, x) 61Cu (180μA proton irradiation, 22MeV, 3.2h) and purified by a ion chromatography method. [ 61Cu]NHAG radiochemical purity was >98% and >99.9% by RTLC and HPLC methods respectively after purification by SPE. [ 61Cu]NHAG was administered into normal and tumor bearing mice followed by bio distribution studies up to 180 minutes. The best tumor accumulation was observed by animal sacrification after 120 min (tumor/muscle and tumor/blood ratios were 25.6 and 3.4 respectively). [ 61Cu]NHAG is a potential PET radiotracer for tumor imaging.