Dyeing fungi: amphotericin B based fluorescent probes for multiplexed imaging

Abstract

The clinically used antifungal polyene amphotericin B was conjugated,viathe mycosamine and the aglycon moieties, to fluorophores. The Cy5 conjugated probe showed selective labelling of fungi in the presence of bacteria, allowing multiplexed imaging and identification of microbial species in a co-culture of fungi and Gram-positive and Gram-negative bacteria.