High Frequency of Multiple Shoots Induction in Paederia Foetida (L.)- A Rare Medicinal Plant

Abstract

Plants have been the eternal source of food and medicine since antiquity in all traditions and cultures. The medicinal plants are considered as one of the most vital components of ecosystem and biodiversity. Tissue culture techniques are usually used for the propagation as well as conservation of medicinal plants. In vitro multiplication of shoot induction and other tissue culture studies is hither unknown in this species. In this study, experiments were designed to develop regeneration protocols for the propagation as well as conservation of a rare medicinal plant, Paederia foetida (L.) The high frequency of multiple shoots was recorded from direct organogenesis of nodal explant. MS medium supplemented with benzyl adenine (4.4µM), kinetin (4.6µM), and 3% sucrose promoted the maximum number of shoots as well as beneficial shoot length. The direct organogenesis in TDZ (2.2µM) containing medium gave maximum percentage of shoots in MS medium from nodal segments compared with that of the other growth regulators. These plants grew normally without showing any morphological variations from the mother plant.