Evaluation of traditional Indian antidiabetic medicinal plants for human pancreatic amylase inhibitory effect in vitro

Abstract

Pancreatic α-amylase inhibitors offer an effective strategy to lower the levels of post prandial hyperglycemia via control of starch breakdown. Eleven Ayurvedic Indian medicinal plants with known hypoglycemic properties were subjected to sequential solvent extraction and tested for α-amylase inhibition, in order to assess and evaluate their inhibitory potential on pancreatic α-amylase. Analysis of 91 extracts, showed that 10 exhibited strong Human Pancreatic Amylase (HPA) inhibitory potential. Of these, 6 extracts showed concentration dependent inhibition with IC 50 values, namely, cold and hot water extracts from Ficus bengalensis bark (4.4 and 125μgmL -1), Syzygium cumini seeds (42.1 and 4.1μgmL-1), isopropanol extracts of Cinnamomum verum leaves (1.0μgmL-1) and Curcuma longa rhizome (0.16μgmL-1). The other 4 extracts exhibited concentration independent inhibition, namely, methal extract of Bixa orellana leaves (49μgmL-1), isopropanol extract from Murraya koenigii leaves (127μgmL-1), acetone extracts from C. longa rhizome (7.4μgmL-1) and Tribulus terrestris seeds (511μgmL -1). Thus, the probable mechanism of action of the above fractions is due to their inhibitory action on HPA, thereby reducing the rate of starch hydrolysis leading to lowered glucose levels. Phytochemical analysis revealed the presence of alkaloids, proteins, tannins, cardiac glycosides, flavonoids, saponins and steroids as probable inhibitory compounds. Copyright © 2011 Sudha Ponnusamy et al.