Comparative effect of amino acids in the production of cyclosporin by solid and submerged fermentations

Abstract

Cyclosporin aids in the differentiation and proliferation of T-cytotoxic lymphocytes that are the main cause of the rejection of organ transplantations. Fungi belonging to the Class Deuteromycetes, Tolypocladium inflation (ATCC 34921) in submerged fermentations, accomplish the production of cyclosporin. In this study, a comparison was made in the yields of cyclosporin in both the solid and submerged fermentations (SmF) with and without the addition of amino acids. A 40% increased yield in cyclosporin was obtained with SSF when compared to the SmF without the addition of amino acids. Though the incubation periods were higher in SSF, an increased yield was achieved with the addition of L-valine (221.6 mg kg-1), L-leucine (206.4 mg kg-1) and L-α-Aminobutyric acid (204.9 mg kg-1). © 2008 Asian Network for Scientific Information.