Correlation of autoantibodies presence detected by ifa-anti-dsdna, ifa-ama and immunoblotting with corresponding data in clinical management of autoimmune diseases

Abstract

Diagnosis and management of patients with SLE (Systemic Lupus Eritematosus), autoimmune hepatitis (AIH), primary biliary cirrhosis (PBC), involves specifi c diagnostic tests, such as IFA-AMA, IFA anti-dsDNA and immunoblotting for the detection of autoantibodies for specifi c autoantigens (mitochondria, dsDNA, M2, LKM-i, LC-i, SLA/LP). We established specifi c correlation between the detected autoantibodies and corresponding clinical fi ndings. The total of 813 serum specimens were probed with IFA-anti-dsDNA, 98 of which tested positive. We also performed dilution analysis to the end point for all the positive specimens. Numerous specimens were tested by IFA, AMA and immunoblotting.