Selective spectrofluorimetric method for the determination of perindopril erbumine in bulk and tablets through derivatization with dansyl chloride

Abstract

Background: Perindopril erbumine is an antihypertensive, which belongs to the category of angiotensin-converting enzyme inhibitors (ACE inhibitors) that inhibit the conversion of angiotensin I to angiotensin II. Methods: A new, selective, and sensitive spectrofluorimetric method was developed for the determination of perindopril erbumine based on the reaction with dansyl chloride in alkaline medium to give a highly fluorescent derivative which was measured at 496 nm after excitation at 340 nm in dichloromethane. The reaction conditions were studied and optimized. Results: Under the optimum conditions, the fluorescence intensity was linear over a concentration range of 1.0 to 21.0 μg/mL (R2 = 0.9997) with a detection limit of 0.242 μg/mL. In order to validate the method, the results were compared with those obtained by a high performance liquid chromatography method. Conclusions: The proposed method was successfully applied to the analysis of perindopril erbumine in pure form and tablets with good precision and accuracy as revealed by t- and F tests. The mechanism of the reaction has also been discussed.