Shoot Culture for Almond and Almond-Peach Hybrid Clones in Vitro1

Abstract

A method for growing almond ( Prunus amygdalus Batsch) and almond-peach hybrid shoots in vitro is described using dormant shoot buds collected from December to February and stored at 3°C. Shoot tips can be in cultured in 0.7 to 0.8% agar, modified Knop’s macroelement mineral solution, 2% sucrose, FeEDTA, microelements and organic supplements of Murashige and Skoog medium, 6-benzyladenine (BA), and light. At 0.1 mg/liter, BA produced shoot elongation; at 1 mg/liter, lateral shoot proliferation. Limited rooting has been obtained.