ANTIBACTERIAL ACTIVITY TEST OF DIFFERENT PARTS OF GLETANG (Tridax procumbens) FROM WEST SUMATERA, INDONESIA

Abstract

The study evaluates the antibacterial activities of different parts of gletang (Tridax procumbens) against Streptococcus mutans and Enterococcus faecalis oral pathogens bacteria. The extracts of different parts were subjected to qualitative phytochemical screening and antibacterial activity. The antibacterial activity of extracts of the plants was investigated using the agar Kirby-Bauer method and chlorhexidine as a positive control. The Minimum inhibitory concentration (MIC) and minimum bacteriocide concentration (MBC) using the ELISA reader micro-dilution method in 96-well microplates. The part of Tridax procumbens was extracted with ethanol 96%. Phytochemical screening showed that the leaf extract contained flavonoids, terpenoids, tannin, and saponin, while stem extract contained flavonoids, terpenoids, tannin, and the flower alkaloids, flavonoids, tannins, and terpenoids. The results showed that % of the leaf, stem, and flower of Tridax procumbens with concentrations of 20, 40, and 60% had antibacterial activity against S. mutans and E. faecalis in the resistant to moderate category. The MIC values of stem, leaf, and flower T. procumbens against S. mutans were 2.5, 10, and 5%, respectively, and MBC values were 10, 20, and 20%, respectively. The MIC values of stem, leaf, and flower gletang against E. faecalis were 5, 10, and 10%, respectively, and MBC values were 10, 40, and 40%, respectively. The highest antibacterial activity was shown in the stem extract of gletang.