Regulation of deinococcus radiodurans RecA protein function via modulation of active and inactive nucleoprotein filament states

Abstract

Background: The RecA protein of Deinococcus radiodurans is essential for extreme radiation resistance. Results: A nucleoprotein state of the DrRecA protein exists that is inactive with respect to ATP hydrolysis. SSB is essential for DrRecA-mediated DNA strand exchange. Conclusion: There are at least two functional states of DrRecA filaments bound to DNA. Significance: A potential new mode of regulation is revealed for DrRecA. © 2013 by The American Society for Biochemistry and Molecular Biology, Inc.