Effects of chilling on respiration and induction of cyanide-resistant respiration in seedling roots of cucumber

Abstract

The effects of chilling stress on respiration and induction of the alternative pathway (AP) were investigated with roots of 3-day-old cucumber seedlings (Cucumis sativus L.) grown at 26 °C and then chilled at 2, 10, or 15 °C for 24, 48, 96, 144, or 192 hours. Oxygen uptake by roots exposed to 2 °C was significantly lower than by 10 or 15 °C-treated roots, and the inhibition of oxygen uptake in the presence of SHAM (salicylhydroxamic acid) increased from 29% in unchilled tissue to 60% after 96 hours of treatment. At 10 and 15 °C, the capacity of the AP was nearly double that of the unchilled control and the 2 °C-treated seedlings. A 24 hours, postchilling treatment at 26 °C resulted in greater oxygen uptake at all temperatures as treatment time increased up to 96 hours. At 2 °C, the capacity of the AP was significantly reduced below the level of the 10 and 15 °C-treated tissue and the untreated control. The activity of the AP became fully activated by 96 hours in roots chilled at 2 °C. Results suggest that the capacity of the AP can be affected by low-temperature treatments, and exposure to 2 °C for up to 96 hours leads to a significant loss in capacity of the AP.