In vivo detection of free radical metabolites

Abstract

During the past two decades, the ESR spin-trapping technique has been used extensively to detect free radicals generated by metabolism of xenobiotic compounds in vitro. Although this ESR technique has proven useful in vitro, it has only recently been applied to drug metabolism in vivo. Spin trapping has successfully been employed to study the metabolism of halocarbons, hydrazines and hydroperoxides in perfused organs and in whole animals. The results of these current studies are reviewed herein. © 1990 De Gruyter