Nitrate uptake and N allocation in Triticum aestivum L. and Triticum durum Desf. seedlings

Abstract

Inter- and intra-species differences in nitrate uptake and N allocation were studied in wheat seedlings. Two collections of wheat cultivars Triticum aestivum and Triticum durum were grown at controlled conditions in hydroponics (773μM NO3-, i.e. 10.8 ppm N-NO3-). At the age of 3 weeks the net rate of nitrate uptake was measured in depletion experiments and it was expressed as μmol NO3- per g of root fresh weight per hour (μmol/g FW/h). Nitrate uptake capacity of the whole root system was expressed as μmol NO 3- per plant per hour (μmol/plant/h). At the same time wheat plants were harvested and analyzed for nitrogen content. In contrast to the net rate of NO3- uptake (3.98-8.57 μmol/g FW/h) the net NO3- uptake capacity of T. aestivum roots (6.37-11.66 μmol/plant/h) significantly differed from T. durum roots (15.26-22.69 μmol/plant/h). Within T. aestivum collection cultivar Roxo exhibits the lowest value in both traits (3.98 μmol NO3-/g FW/h and 6.67 μmol NO3-/plant/h). By contrast Strela was characterized by relatively low NO3- uptake rate (5.47 μmol/g FW/h) and the highest NO3- uptake capacity (11.66 μmol/plant/h). Intra-species differences in T. durum group were not significant. In both species about 70% total nitrogen was found in shoot. Statistically significant differences in nitrogen content and its allocation were affected by growth rate in early stages of development.