In vitro cultivation and regeneration of bean (phaseolus vulgaris l.)

Abstract

Possibilities and limitations concerning in vitro regeneration of bean (Phaseolus vulgaris L.) based on the existing literature data were discussed. Some parallels between traditional breeding methods applied for creation of new bean cultivars with valuable agricultural traits and in vitro biotechnological approaches was made. Authors present the next main areas of tissue culture that are more possible to be used for bean improvement: A) Micropropagation, and morphogenesis; B) Somatic tissue and cell manipulations; C) Protoplast isolation; D) Anther culture and E) Embryo rescue. A conclusion along with original hypnotizes concerning difficult regeneration, low efficiency and repeatability of biotechnological methods in Phaseolus vulgaris was presented at the end of the manuscript. © 2003 Taylor and Francis Group, LLC.