Studies on mycotoxin analysis using immunoaffinity column (2002 Japanese Association of Mycotoxicology Achievement Award)

Abstract

The traditional anal. of mycotoxins in food and feed required considerable time, large vol. of solvents and often, special techniques. To overcome these problems, the authors developed the new anal. method for ochratoxin A (OTA) using the immunoaffinity column (IAC) specific for ochratoxin A (OTA) in 1990. The advantage of this method was one-step removal of interfering substances from samples. Moreover, the detection limit of 0.05 micro g/kg for OTA in coffee beans was achieved. Using the IAC cleanup and HPLC with fluorometric or electrochem. detection, the surveys of aflatoxins (AFs) and OTA in green and roasted coffee beans, AFs in spices, and AFs, OTA, zearalenone (ZEN) and beta -zearalenol (beta -ZEL) in beers were conducted. With the use of IACs in the cleanup steps, the parts-per-trillion ranges of mycotoxins were detectable. The detection limits were 0.002micro g/kg for AFB1 in coffee beans and spices, and 0.05micro g/kg for OTA in coffee beans, 0.0005micro g/L for AFB1, 0.0010micro g/L for OTA, 0.020micro g/L for ZEN and beta -ZEL in beer samples. This paper also describes the effects of the elimination of fungi, AFB1 and OTA in green coffee beans by handpicking. on SciFinder (R)