Experimental Investigation on the Effects of Audible Sound to the Growth of Aspergillus Spp

Abstract

This paper discusses the effect of sound on the growth of fungus, Aspergillus spp. Aspergillus was cultured on Potato Dextrose Agar (PDA) and Potato Dextrose Broth (PDB) and exposed to sound waves for five hours per day for three days. This experiment was observed for three days and data were recorded everyday after the exposure. Four parameters were used to measure the growth of fungus which were colony forming units per ml, optical density, length of germ tube and the diameter of the colony. The results showed that sound waves have effects on the growth of Aspergillus. When Aspergillus was exposed to sound waves with frequencies of 5 kHz, 10 kHz and 15 kHz, the growth was affected by the different frequency. The higher the frequency, the higher the growth inhibition found on Aspergillus. All the three frequencies inhibited the growth of Aspergillus compared to the control (No exposure to sound) and the maximum inhibition occurred at the frequency of 15 kHz.