Protoplasts of Marchantia polymorpha Are Stabilized by Low Concentrations of Cellulase in the Medium During the Early Stage of Culture.

Abstract

Protoplasts isolated from callus tissues of Marchantia polymorpha L. were cultured in medium with cell wall degradating enzymes containing cellulase activity. Survival of Protoplasts decreased rapidly in medium without enzymes, whereas addition of low concentrations (0.01-0.1%) of enzymes prevented decrease in protoplast survival. Protoplasts precultured in medium with enzymes for 3 days showed a high rate of division when transferred to medium for division of protoplast-derived cells. Possible involvement of the regenerated cell wall in destabilization of cultured Protoplasts was discussed.