Search for genes - Potential markers of aggressiveness and metastasis for human prostate cancer

Abstract

Aim. An investigation of the expression levels of 84 cancer-related genes, involved in the main molecular mechanisms of carcinogenesis, in androgen-independent (AI) cell line PC3 compared to androgendependent (AD) cell line LNCaP to find markers of the prostate cancer (PC) aggressiveness and metastasis. Methods. Commercial Q-PCR array was used to determine the genes expression levels. Results. Thirty six genes from the total 84 examined changed expression levels in PC3 cell line compared to LNCaP cell line by 4-fold or more times. Increased expression of the genes of angiogenesis (PDGF, TGFB1, THBS1), invasiveness and metastasis (MET, MMP1, PLAU) and antiapoptotic factors (BCL2, BCL2L1) were detected. Seven genes (MET, MMP1, PLAU, SERPINE1, EPDR1, TGFB1, VEGFA) were selected as potential markers of the PC aggressiveness and metastasis. Conclusions. The expressions of the genes of invasiveness and metastasis, cell cycle control and apoptosis undergo the greatest changes during transition from AD to AI type of the prostate cancer. The selected genes could serve as potential markers of invasiveness, metastasis and require further investigation. © Institute of Molecular Biology and Genetics, NAS of Ukraine, 2013.