Brine Shrimp Lethality Test of Methanolic Extracts from Four Different Marine Biota in Lampung Province, Indonesia

Abstract

This study aims to test the LC50 toxicity activity of brine shrimp using four samples of marine biota. The LC50 toxicity activity has the potential as an anti-bacterial, anti-fungal, and anti-cancer agent that needs to be studied further before being used as an industrial-scale natural product, this study is basic research on the brine shrimp lethality test. The test was carried out using the Brine Shrimp Lethality Test (BSLT), which analyzes the number of deaths of shrimp larvae to determine the level of toxicity of an ingredient. The marine biota samples used were Rhizopora sp., Sargassum sp., Halimeda sp., Diadema setosum. The manufacturing stage begins with making extracts and hatching artemia, then toxicity tests are carried out within 24 hours with different concentrations of sample solutions. The results of this research show that the sample extracts that have the highest to lowest toxicity values respectively are Rhizopora sp., Sargassum sp., Halimeda sp., Diadema setosum. with a value of 870.96 µg/mL; 745.05 µg/mL; 697.97 µg/mL; 575.4 µg/mL.