Establishment of FTIR Database of Roselle Raw Material Originated From Western Coastline in Peninsular Malaysia

Abstract

Herbs from different geographical regions may differ qualitatively and quantitatively , hence it is crucial to determine the active components of herbs from different regions and build a reference database. This study focused on the database establishment for the authentication of the raw material of roselle (Hibiscus sab-dariffa) collected at seven selected locations of the western coastline in Peninsular Malaysia. The validation on the unknown sample at the end of the study is to verify the accuracy of the established database. The inter-material distance (IMD) was presented as the mean distance of each sphere created by each batch of data from different locations. They were clustered with different folders and discriminated by Soft independent modelling by class analogy (SIMCA) algorithm. All materials from seven farms achieved 100% separation rate. The average IMD of these seven locations was 9.04. The FTIR techniques established in this study can be used to distinguish the geographical origin of the selected H. sabdariffa farm samples.