Noninvasive genetic sampling of endangered muriqui (Primates, Atelidae): Efficiency of fecal DNA extraction

Abstract

The muriqui (Brachyteles) is one of the most endangered primates in the world, however little is known about the viability of the remaining populations. We evaluated the technique of extracting DNA from wild muriqui feces for PCR applications. In order to determine the effect of the DNA in subsequent amplifications, we analyzed five different extracts. The importance of the recommended BSA and the HotStarTaq DNA polymerase was tested. The minimal conditions to successfully amplify highly degraded fecal DNA were determined, showing that the recommended re-agents are not required. We envision that this method may be useful in further conservation management studies. Copyright by the Brazillian Society of Genetics.