Fundamental limits in single-molecule orientation measurements

Abstract

Directionality inherent in the polarization of light affords the means of performing robust dynamic orientational measurements of molecules and asymmetric scatterers. In this paper, the precision with which measurements of this kind can be made is quantified for a number of common polarizationbased measurement architectures using a metric derived from Fisher information. Specifically, a fundamental limit of 0.5 radian per detected photon (on average) is found, thus highlighting the importance of maximizing photon numbers by correct fluorophore selection. Informational dips, whereby measurement precision is degraded, are shown to arise in many realistic measurement scenarios, particularly for inference from null readings. The severity of these precision losses is therefore considered, and it is shown to decrease with increased system redundancy. Contamination of measured data from coherently and incoherently radiating extraneous sources, furthermore, causes a loss of precision. Analytic and numerical results are hence also presented in this vein. © IOP Publishing Ltd and Deutsche Physikalische Gesellschaft.