Cytotoxicity, scavenging and lipid peroxidation - inhibiting activities of 5,3',4'-trihy-droxy-7-methoxyflavanone isolated from Haplopappus rigidus

Abstract

The cytotoxicity, scavenging and lipid peroxidation - inhibiting activities of 5,3',4'-trihydroxy-7-methoxyflavanone, isolated from Haplopappus rigidus Phil.(Asteraceae), were evaluated in a series of in vitro assays involving free radicals (ABTS, DPPH), ascorbate - induced rat liver microsomal lipid peroxidation, and the cytotoxicities to three human tumor cell lines. 5,3',4'-trihydroxy-7-methoxyflavanone, at concentration of 0.3 mg/mL, elicited an 97.4 % scavenging effect on DPPH; TEAC 1.35mM on ABTS, preventing microsomal lipid peroxidation IC50 1.8 μM and ED50 3.23 - 5.66 μg/mL against three human tumor cell, indicating that the flavanone 5,3',4'-trihydroxy-7-methoxyflavanone was effectively cytotoxic, as well as free radicals scavenger and a good inhibitor of iron/ascorbate induced lipid peroxidation in a dose dependent manner.