The value of local malaria strains for serological studies: Local strains versus Palo Alto reference strain

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Abstract

Background: The standardization of the type of crude Plasmodium falciparum extracts for assays to evaluate the overall anti-blood-stage immune response in humans may be beneficial to malaria pre-elimination programmes. However, there is no consensus on which strain is appropriate for routine analyses. This study aimed to compare the responses of malaria IgG antibodies in serum collections from Dielmo and Ndiop to crude extracts of merozoites and schizonts of local and reference strains of P. falciparum. Methods: Malaria antibodies were evaluated using serological tests for exposure to three local strains (0703, F15 and F16) and the P. falciparum reference Palo Alto strain (PA). A total of 218 sera collected in 2000 from inhabitants of the villages of Dielmo and Ndiop were used: 142 from Dielmo and 76 from Ndiop. The biological collection was used to evaluate by ELISA the prevalence of IgG antibodies against crude merozoite and schizont extracts. The genetics of the local and reference strains were compared. Results: There was genetic divergence between strains 0703, F15, F16 and PA. IgG responses against local and reference strains correlated well (0.6 to 0.8; p∈

Figures

  • Fig. 1 PCR analysis of the polymorphic region of msp2 (block 3) of different P.falciparum isolates from Dielmo (0703), Dakar (F15 and F16) and Uganda (PA). The DNA size marker is a 100 bp ladder
  • Fig. 2 Correlation between Ab responses to schizont crude extracts
  • Fig. 3 Correlation between Ab responses to merozoite crude extracts
  • Fig. 4 Comparison of antibody responses against schizont crude extracts ( [Foundation Pasteur P. falciparum 16], 0703 [P. falciparum 0703]. Measurem the villages of Dielmo and Ndiop
  • Fig. 5 Comparison of antibody responses against merozoite crude extracts. (PA [Palo Alto], F15 [Foundation Pasteur P. falciparum 15], F16 [Foundation Pasteur P. falciparum 16], 0703 [P. falciparum 0703]. Measurements were performed with sera collected in 2000 from inhabitants of the villages of Dielmo and Ndiop
  • Fig. 6 Cross comparison of antibody responses between Plasmodium falciparum schizonts and merozoites. (PA [Palo Alto], F15 [Foundation Pasteur P. falciparum 15], F16 [Foundation Pasteur P. falciparum 16], 0703 [P. falciparum 0703]. Measurements were performed with sera collected in 2000 from inhabitants of the villages of Dielmo and Ndiop
  • Fig. 7 Positive responders to crude extracts of four Plasmodium falciparum strains (PA [Palo Alto], F15 [Foundation Pasteur P. falciparum 15], F16 [Foundation Pasteur P. falciparum 16], 0703 [P. falciparum 0703]. Measurements were performed with sera collected in 2000 from inhabitants of the villages of Dielmo and Ndiop
  • Table 1 Percentage of positive responders to crude extracts of four Plasmodium falciparum strains

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APA

Diop, F., Diop, G., Niang, M., Diouf, B., Ndiaye, D., Richard, V., & Balde, A. T. (2015). The value of local malaria strains for serological studies: Local strains versus Palo Alto reference strain. Malaria Journal, 14(1). https://doi.org/10.1186/s12936-015-0734-z

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