Inhibitory Effect of miR-339-5p on Glioma through PTP4A1/HMGB1 Pathway

Abstract

Objective. Finding miR-339-5p inhibitory functions in glioma through PTP4A1/HMGB1 pathway. Methods. From May 2020 to August 2021, 20 glioblastoma and para cancer tissues were chosen for qRT-PCR analysis. The miR-NC, miR-con, miR-339-5PMIMIC, and miR-con + groups were transfected into human glioma U251 cells. The capacity of cell vascular-like structure construction was found by simulating angiogenesis, and the ability of cell movement was examined by cell scratching. The twofold luciferase reporter gene method determined that miR-339-5p targets PTP4A1, and the protein expression levels of PTP4A1 and HMGB1 were examined using Western blot. Results. MiR-339-5P expression was substantially lower in cancer samples than noncancer samples (P<0.05). PTP4A1 expression in cancer samples was higher than in healthy controls (P<0.05). The miR-339-5p group produced significantly less vascular-like structures than the NC and miR-con groups (P<0.05). The miR-339-5p group lowered the invasive index and migratory rate of U251 cells (P<0.05). PTP4A1 inhibited the luciferase activity of the pTP4A1-WT reporter gene (P<0.05) but not the PTP4A1-MUT (P>0.05). The miR-339-5p group had lower protein levels of PTP4A1 and HMGB1 than the NC and miR-con groups (P<0.05). The development of vascular-like structures was substantially more significant in the miR-con +PTP4A1 group than in the miR-con and miR-339-5p +PTP4A1 groups (P<0.05). In terms of migration and invasion index, there was a substantial difference between the miR-339-5p +PTP4A1 and the miR-con +PTP4A1 groups (P<0.05). The miR-con +PTP4A1 group had a greater migration rate and invasive index than the miR-con and miR-339-5p +PTP4A1 groups (P<0.05). Conclusion. MiR-339-5P inhibits angiogenic mimicry, migration, and invasion of brain glioma U251 cells by inhibiting the PTP4A1/HMGB1 signal pathway.