A study on saccharification and fermentation of dry-pulverized ligoncellulosic biomass

Abstract

Bio-ethanol production from Japanese cedarwood using an enzyme and yeast cells was investigated. Japanese cedarwood was pulverized into powder by a high impact-pulverizing mill called inner power ring mill. The enzymatic saccharification examinations were carried out on solids concentrations of 13.5, 18, 22.5 and 27w/v% using acetic buffer solution of 200ml, a powder pulverized into 20μm in average diameter and an enzyme dosage of 5wt% per solids of powder. Ethanol fermentations with three fermentation states as fermentation without residual solids, fermentation with residual solids and simultaneous saccharification and fermentation (SSF) were carried out by yeast of Saccharomyces cerevisiae on the enzymatic saccharification liquid. As the results, the bio-ethanol yield of SSF was reached a high yield in a short time in this study. © 2012 The Japan Society of Mechanical Engineers.