PENGARUH STRES AKUT TERHADAP KADAR GULA DARAH MENCIT (Mus Musculus) DENGAN PERLAKUAN EKSTRAK ETANOL ALGA COKELAT (Sargassum sp.)

Abstract

Stress is a non-specific response in the body to conditions or conditions that occur as a result of exposure to stressors. High stress usually has a negative impact on the body, one of which is increasing blood sugar levels. Brown algae is one of the traditional marine plants and contains secondary  metabolite compounds such as alkaloids, flavonoids, and steroids which are  antidepressants  so  that  they  can  prevent  the  increase  in  blood  sugar  levels.  This  study  aims  to determine  the  effect  of acute  stress  on  blood  sugar  levels  of  mice  by  giving  ethanol  extract  of  brown  algae.  This  research  is  a  true  experimental research design with pre test and post test control group design. 30 mice were divided into normal control (KN), negative control (K-), positive control (K +) groups, ethanol extract of brown algae with a concentration of 2.5% (P1) and 5% (P2). The study was started by measuring the initial blood sugar levels in all groups, then groups K-, K +, P1, and P2 were given stress  treatment  with  the  FST  method  for  6  minutes  for  7  days.  Group  K  +  was  given  alprazolam at  a  dose  of  0.2  ml  at  1 hour before treatment, while P1 and P2 were given brown algae at 30 minutes before treatment. After the treatment, it was followed by measurement of the final blood sugar level. The data from the observations were analyzed using the dependent T-test  with  a  confidence  level  of  95%.  The  results  showed  that  the  post-test  blood  sugar  levels  in  the  P1  and  P2  groups experienced an increase, but the increase was not significant. The results also showed that the significance values (2-tailed) in the P1 and P2 groups were 0.772 and 0.392 (sig> 0.05), meaning that it could be concluded that there was no effect of giving ethanol extract of brown algae on the prevention of increasing blood sugar levels after acute stress.