An electrochemiluminescent magneto-immunosensor for ultrasensitive detection of hs-cTnI on a microfluidic chip

Abstract

HIGHLIGHTS • An integrated microfluidic chip is fabricated to confine immunomagnetic beads at the electrode surface, leading to improved electrochemiluminescent efficiency and increased sensitivity in electrochemiluminescent immunoassays. • The combination of single-walled carbon nanotubes with the excellent electrooxidation ability of TPA and SiO2 nanoparticles as carriers loading a large amount of [Ru(bpy)3]2+ enables a sensitivity improvement of at least two orders of magnitude. • The electrochemiluminescent magneto-immunosensor exhibits an ultralow detection limit of 8.72 fg/ml, excellent selectivity and reproducibility, and satisfactory recoveries in human serum.