Enhanced Antibacterial Activity of Manuka Honey with Higher Methylglyoxal Concentration Against Staphylococcus aureus: in vitro Study

Abstract

Objective: This study aimed to investigate the antibacterial activity of two types of Manuka honey with varying concentrations of methylglyoxal (MGO), 70 and 1050 mg/kg against S. aureus in vitro. Methods: Two types of Manuka honey were purchased and tested for their antimicrobial activities. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were determined using the broth tube dilution method. Results: The results revealed that both concentrations of Manuka honey exhibited antibacterial activity against S. aureus, with the higher concentration (1050 mg/kg) exhibiting enhanced antibacterial activity. The MIC was determined to be 100% v/v (undiluted) for the initial concentration and 1:2 (50% v/v) for the higher concentration. The MBC was not reached at the dilution level used in this experiment for the initial concentration, but it was inferred to be at a dilution of 1:2 for the higher concentration. Conclusion: These findings suggest that Manuka honey with a higher concentration of MGO may be more effective in inhibiting the growth of S. aureus and potentially killing bacteria present. The results of this study highlight the potential use of a high concentration of Manuka honey as an alternative antimicrobial agent against S. aureus. Further investigation into its efficacy in various clinical settings is required.