Effect of Multiyear Biodegradable Plastic Mulch on Soil Microbial Community, Assembly, and Functioning

Abstract

The increasing use of biodegradable plastic mulch like polybutylene adipate terephthalate (PBAT) has raised concerns about its long-term environmental impact. In this study, we investigated the effects of multiyear PBAT mulch application on bacterial and fungal communities, assembly mechanisms, and key ecological functions. The microbial community diversity and composition were significantly altered after multiyear biodegradable plastic mulching. We observed that PBAT treatment enriched specific bacterial genera, such as Pantoea, potentially involved in plastic degradation, and fungal genera like Cephaliophora and Stephanosporaceae, which may play a role in organic matter decomposition. A null model analysis revealed that bacterial community assembly was largely shaped by deterministic processes, with stronger environmental selection pressures in PBAT-treated soils, while fungal communities were more influenced by stochastic processes. In addition, multiyear PBAT mulch application also impacted the functionality of the soil microbial communities. PBAT exposure enhanced biofilm formation in aerobic bacteria, promoting aerobic degradation processes while also reducing the abundance of stress-tolerant bacteria. Additionally, PBAT altered key microbial functions related to carbon, nitrogen, and sulfur cycling. Notably, the fungal communities exhibited functional shifts, with an increase in saprotrophic fungi being beneficial for nutrient cycling, alongside a potential rise in plant pathogenic fungi. These findings underscore the multiyear ecological impacts of biodegradable plastics, suggesting microbial adaptation to plastic degradation and changes in key ecological functions, with implications for agricultural sustainability and bioremediation strategies.