Cell-sized polyelectrolyte capsules functionalized with a redox-driven proton pump protein were assembled for the first time. The interaction of polyelectrolyte microcapsules, fabricated by electrostatic layer-by-layer assembly, with cytochrome c oxidase molecules was investigated. We found that the cytochrome c oxidase retained its functionality, that the functionalized microcapsules interacting with cytochrome c oxidase were permeable and that the permeability characteristics of the microcapsule shell depend on the shell components. This work provides a significant input towards the fabrication of an integrated device made of biological components and based on specific biomolecular functions and properties.
Pastorino, L., Dellacasa, E., Noor, M. R., Soulimane, T., Bianchini, P., D’Autilia, F., … Ruggiero, C. (2014). Multilayered polyelectrolyte microcapsules: Interaction with the enzyme cytochrome c oxidase. PLoS ONE, 9(11). https://doi.org/10.1371/journal.pone.0112192