THU0494 BACK PAIN, SPINE OSTEOARTHRITIS AND ‘CANDIDATE GENES’ POLYMORPHISM

Abstract

Background: Low back pain (LBP) and spine osteoarthritis are among the leading health-related causes of disability and reduction in patient quality of life. More recent work suggested that the factors that lead to disc degeneration may have important genetic components. Genetic predisposition has been confirmed by recent findings of associations between degeneration and gene polymorphisms of matrix macromolecules. Objectives: Evaluation of genetic polymorphisms of genes collagen 1A1 (COL1A1), interleikin 1β (IL-1β), interleikin 6 (IL-6), vitamin D receptor (VDR) in patients with LBP, associated with spine osteoarthritis. Methods: We examined 33 patients (men-17, women-16, middle age 28, 7±3,77) with LBP end onset, verified by magnetic resonance imaging (MRI) and 15 controls was carried out. Assessed the intensity of pain syndrome on visual analog scale (VAS, mm) and the nature of its current (IASP, 1994). Polymerase chain reaction and restriction fragments length polymorphism was used to detect the polymorphism of COL1A1 (rs 1800012), IL-1 (rs 1143627), IL-6 (rs 1800795), VDR (rs 1544410). Results: The severity of the pain (VAS) average was 60 mm, all the patients revealed chronic option currents pain syndrome. In 86 % of patients detected changes disc in the form of protrusions and hernias, 17 % of patients had a characteristic reducing the height of the intervertebral disc of lower height disc and osteophytes, arthritis facet joints identified in 20 % of patients in 34% of patients diagnosed changes type Modic II. In 83% of patients with LBP identified homozygous variant allele (GG) Col1A1 gene. The absence of T-alleles in the gene IL 1β in the group of patients was associated with severity of the disease. Carriage of allele in heterozygous AG found in 54% of patients of the main group and was not observed in the control that requires further accumulation of facts. Identified association GG-genotype of IL-6 clinical and instrumental signs of the syndrome Modic-II. Conclusion: These findings may be the reason for the patient-specific approach to diagnosing and treatment of back pain. It proves the necessity of research of genetic polymorphisms in patients with spine osteoarthritis.