Embriogenesis somatik pada kultur in vitro daun kopi robusta (coffea canephora var. Robusta chev.)

Abstract

This research aims to induce tissue cells of robusta coffee leaves (Coffea canephoravar. Robusta Chev.) for embryogenesis through the addition of growth regulators kinetin and 2,4-D.The growth medium used is a solid Murashige-Skoog medium (MS). The added growth regulators areA = 10-7 Kinetin without 2,4-D, E = 5 x 10-6 M Kinetin and 2.5 x 10-5M 2,4-D; H = 5x10-6M Kinetin and5x10-5 M 2,4-D, I = 7.5 x 10-6M Kinetin and 5x10-5 2,4-D. Explants used were the second leaf from topbranch ortotroph of coffee plants with a size of about 0.5 x 1.0 cm. Observations were made on thepercentage of live explants, explant growth response including the formation of callus, organogenesis,and embryogenesis. The results showed that the planted explants are 100% alive, the growthresponse in the form of direct somatic embryogenesis occurred on the addition of Kinetin 10-7 without2,4-D. Other Treatment, E produced a response in the form of greenish compact callus, while twoother treatments, H and I, form whitish crumb/fragile structured callus. Thus, it was concluded that invitro culture of leaf tissue of Robusta coffee (Coffea canephora var. Robusta Chev.) on Murashige-Skoog (MS) medium with the addition of kinetin growth regulators and 2,4-D at different concentrationsproduce higher different growth rate. Response of growth that occurs is in the form of direct somaticembryogenesis, compact and crumb/fragile structured callus.