The derivation of sublines of low and high malignancy from rat and mouse tumours

Abstract

Sublines of differing malignancy have been prepared from 2 tumours-a carcinogen induced rat tumour and a spontaneous tumour in AKR mice-by tumour progression in isogeneic animals. The rat tumour sublines of high (WBP1(V)) and low (WBP1(A)) malignancy were ascitic and produced mean death times of 15·3 days and 31·1 days respectively when 4·5 × 107 cells were injected intraperitoneally into rats. The mouse tumour sublines of high (K12 (V)) and low (K12(A)) malignancy were easily dispersable tumours of the mesenteric lymph nodes and produced mean death times of 11·6 days and 25·1 days respectively when 1 × 106 cells were injected intraperitoneally into AKR mice. We are grateful to Miss S. M. Christie, Miss G. R. Fanstone and Mr. M. S. Macbeth for excellent technical assistance. This work was supported by the British Empire Cancer Campaign for Research. © 1968, The British Empire Cancer Campaign for Research. All rights reserved.