Functional isolation of staurosporine binding protein kinases using CCMS technology

Abstract

Capture Compound mass spectrometry (CCMS) is an innovative technology to reduce biological sample complexity by selective isolation of targeted protein or enzyme families. Small synthetic molecules (Capture Compounds™) are used to interrogate native proteins. Using staurosporine as a selectivity function for a kinase-specific Capture Compound™ enables efficient complexity reduction of the proteome and allows discovery, isolation and profiling of functional kinases in biological samples. Kinase-specific Capture Compounds™ are available as ready-to-use caproKits™ for proteomic research.