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Identification of contaminating fungal DNA sequences in Zymolyase

Journal of Clinical Microbiology (1999) 37(3) 830-831
DOI: 10.1128/jcm.37.3.830-831.1999
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Abstract

When different preparations of Zymolyase were included in the pretreatment protocol of a panfungal PCR assay using a primer system for the 18S rRNA gene, an amplification product occurred in negative controls. The amplified fragment showed 100.0% sequence identity to the Saccharomyces sensu stricto complex and Kluyveromyces lodderae. Lyticase, lysing enzymes, and proteinase K appeared to be free from fungal DNA.

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Rimek, D., Garg, A. P., Haas, W. H., & Kappe, R. (1999). Identification of contaminating fungal DNA sequences in Zymolyase. Journal of Clinical Microbiology, 37(3), 830–831. https://doi.org/10.1128/jcm.37.3.830-831.1999

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